Andranik Ivanov and Sebastian Meczmack (Prof. Rajewsky's . She also enjoys amateur photography. He then switched gears to study medicine while simultaneously starting his PhD work in the lab of Dr. Lena Claesson-Welsh at Uppsala University. Are circRNAs involved in neurodevelopmental and neurodegenerative diseases? Despite the importance of antibodies in biology study, antibodies are not available for most fly proteins. Eric studied how the interaction of cell surface proteins expressed by different cell types can regulate blood vessel formation in normal cells and tumors. Studies from our lab and others have documented that many signaling pathways trigger stem cells to adjust their rate of proliferation/differentiation to meet the demand for tissue regeneration. He also enjoys playing handball and online chess. * Corresponding authors. *, Bartok O. We have taken a number of approaches to address the spatial and temporal regulation of signaling pathway activities in the gut epithelium. Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer. He went to college in Malaysia before moving to Philadelphia. With this advance, we hope to enable the mosquito community to easily perform genome-wide loss- or gain-of-function screens in cells, for example to discover entry and infection mechanisms of pathogens and drugs, as well as to aid in the functional annotation of mosquito genomes. as achieved by mapping signal pathway activities in various cell types), and the physiological roles of various cells. Molecular Neurobiology and RNA metabolism. Nogues G., Kadener S., Cramer P., Bentley D. and Kornblihtt A.R. Transcriptional activators differ in their abilities to control alternative splicing. neta.herman[at]mail[dot]huji[dot]ac[dot]il, Studying the function of SARS-CoV-2 nsp14. Don't wanna be here? Verified email at brandeis.edu - Homepage. What are the general mechanism of the circadian clock to deal with genetic and/or environmental perturbations? Moreover, in 2019, we were funded to function as the Drosophila Research and Screening Center-Biomedical Technology Research Resource (DRSC-BTRR), which focuses on development of new screening and other technologies. Cramer P., Cceres J.F., Cazalla D., Kadener S., Muro, A., Baralle F. and Kornblihtt A. Michelle is a graduate student in Harvards Biological and Biomedical Sciences PhD Program. CircRNAs expression levels are not correlated with the expression of their linear isoforms, indicating a potentially widespread layer of previously unknown gene regulation. We previously developed a recombination-mediated cassette exchange (RMCE) system that enables pooled CRISPR screening in Drosophila cells, as well as bioinformatics tools for identifying sgRNA designs for mosquitos and mosquito cell lines. Interestingly, JAK/STAT signaling in the muscle or fat body directly triggers the expression of ImpL2, which in turn impairs local insulin signaling and its associated energy balance. Second, we identified 53 transcription factors, specifically expressed or enriched in the ISCs, and are characterizing how they cooperate to integrate signal inputs. Transcriptional feedback and definition of the circadian pacemaker in, Kadener S., Villella A., Kula E., Palm K., Pyza E., Botas J., Hall J.C. and Rosbash M. Neurotoxic protein expression reveals connections between the circadian clock and mating behavior in. From 2002 to 2008, Sebastian Kadener was a postdoc working in the Michael Rosbash laboratory. Third, more recently we used single-cell RNAseq (scRNAseq) to describe all the cell types and their transcriptomes in the Drosophila gut. Title. Selected for Faculty of 1000 (Factor 9 Exceptional). CRISPR-generated mutant cell lines, in combination with RNAi, provide a robust platform for combinatorial screening. Clonally stable VK allelic choice instructs IgK repertoire. Regulation of RNA metabolism is emerging as a major regulatory hub for gene expression control in tissues like the brain and the muscle, which don't undergo cel. What are the key functions of these molecules in the brain and during aging? Pvf1 produced by yki-gut tumors activates MEK/ERK signaling and enhances catabolism in multiple tissues. As an underlying mechanism, the balance or critical reaction hypothesis have been proposed. Dana joined the Bandopadhayay Lab in 2021 where is she working to apply single-cell methods to evaluate the heterogeneity of pediatric gliomas, and to understand spatial associations. This dual reporter can be used in either fixed or live tissues to monitor spatio-temporal activity of signaling pathways at an unprecedented resolution. Fsica, Tcnico Sup. Biogenesis and functions of circular RNAs. *, Afik S., Menet J., Wolfson V., Weissbein U., Haimovich D., Gafni C., Friedman N., Rosbash M. and Kadener S. . What are the neural and molecular basis of temperature adaptation and compensation? Additionally, many of the mechanisms by which growth factortriggered signaling events intersect with cell metabolism, which is regulated by nutrients, hormones and stress, remain to be identified. While at Caltech, Adam studied luminescent ruthenium complexes that function as light switch probes for DNA mismatches. SRCP: a comprehensive pipeline for accurate annotation and quantification of circRNAs. Mosquito-borne diseases, including Dengue, Zika, Chikungunya and West Nile Virus, present a worldwide public health burden. Hanan M., Soreq H. and Kadener S. CircRNAs in the brain. ( a) Simplified overview of three stages of the gypsy retrotransposon life cycle, (i) parent insertion in the genome, (ii) the transcribed RNA (genomic RNA), and (iii) the new copy inserted in the genome. As part of his bachelors program, he joined the group of Prof. Tony Kourzarides at the University of Cambridge in 2019 as a visiting undergraduate student to investigate the role and pharmacological inhibition of histone acetyl transferases in leukemia. M.Sc., The University of Buenos Aires, Buenos Aires, Argentina Ph.D., The University of Buenos Aires, Buenos Aires, Argentina. Lacadie S.A., Tardiff D.F., Kadener S. and Rosbash M. In vivo commitment to yeast cotranscriptional splicing is sensitive to transcription elongation mutants. I am studying potential applications of the CRISPR/Cas13 system. Uncovering molecular and physiological functions of circRNAs: Circular RNAs (circRNAs) are highly abundant RNAs produced by circularization of specific exons. Control of alternative pre-mRNA splicing by RNA Pol II elongation: faster is not always better. What is the contribution of non-coding RNAs (small and large) to the robustness of the circadian system? Fun fact: One of the first things Jens did when he arrived in Boston was to buy a bike. Pathways that are misregulated in animals that develop organ wasting in the context of these various tumor models are identified using a combination of transcriptomics, e.g. Using the DRSC screening platform, the functions of the ~15,000 predicted Drosophila genes can be systematically analyzed to address questions in cell signaling, cell morphology, host-pathogen interactions, ion channel function, and many other topics. Selected for Faculty of 1000 (Factor 3 Recommended). * Corresponding authors. Site developed by Health Communication Core, April received her BS in Cellular and Molecular Biology from Beloit College in 2017, where she worked in the lab of Dr. Amy Briggs studying the role of. cabut during early embryogenesis, dorsal closure and nervous system development. Chem. Acquiring answers to these fascinating questions requires a deep understanding of the mechanisms by which cells integrate signals received from their inner selves, neighboring cells, other organs and the environment. Kadener S., Fededa J.P., Rosbash M. and Kornblihtt A.R. In addition to ImpL2, we have also identified two additional factors produced from yki-gut tumors that contribute to tissue wasting: the Pvr receptor tyrosine kinase ligand Pvf1 and the IL6-like cytokine Unpaired 3 (Upd3). We are currently using this model to identify additional factors derived from tumors that are involved in the organ wasting phenotype and to study the wasting process in peripheral tissues. Joined June 2011. Fun fact: In his native language of Urdu, Shers full name translates to lion/tiger brave. In a collaboration with Sebastian Kadeners lab at Brandeis, we generated a new set of PspCas13b and RfxCas13d expression constructs that can be used to target RNA in cells and in vivo. One point of focus for DRSC-BTRR efforts is establishment of CRISPR pooled screening in cell lines from mosquito vectors of infectious diseases. * Corresponding authors. SRCP: a comprehensive pipeline for accurate annotation and quantification of circRNAs. Strikingly, these phenotypes are reminiscent of the pathophysiology of cystic fibrosis (CF), as in CF the non-functional CF transmembrane conductance regulator (CFTR) increases ENaC activity resulting in chronic dehydration of the intraluminal surface liquid in organs such as kidney, colon, lung and sweat glands. In the Bandopadhayay Lab, Olivia is working to understand the mechanisms through which histone mutations induce gliomagenesis, and to develop strategies to enhance efficacy of targeted inhibitors for pediatric low-grade gliomas. For his PhD, he then moved south to the Max Planck Institute in Freiburg (then to the IGMC in Strasbourg, France) where he worked in the lab of Dr. Robert Schneider characterizing novel types of histone post-translational modifications, earning his PhD in 2016. NanoTags: One of the key reagents to understand protein expression and function are antibodies. For example, we showed that circRNA biogenesis competes with canonical splicing, showing that circRNAs can function incisas RNA traps. 178: Two of these RNAs act as miRNA sponges, but no function is known for the thousands of other circRNAs found in species across the animal kingdom. In a collaboration with Sebastian Kadeners lab at Brandeis, we generated a new set of PspCas13b and RfxCas13d expression constructs that can be used to target RNA in cells and in vivo. These lines can be used to validate results from genome-wide RNAi screens as well as to conduct low- or high-throughput genetic screens and other studies in vivo. Anna developed a fascination for cancer biology while working towards her bachelors degree at Lund University, Sweden. Software. Alternative splicing regulates biogenesis of miRNAs located across exon-intron junctions. using proximity labeling to identify all proteins secreted by the tumors. Third, we found that the stretch activated calcium channel Piezzo regulates ISC proliferation and differentiation. I am researching the role of circular RNAs (circRNAs) in aging processes in Drosophila melanogaster. What are the key functions of these molecules in the brain and during aging? We have developed a series of bioinformatics tools that provide the research community with well-designed, user-friendly resources that impact research at all stages, from project design to data analysis and integration. For example, little is understood about how the simultaneous growth and differentiation of different tissues and organs are coordinated and how the development of different cell types and tissues is integrated within an organ. . Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer. Michael earned a BA in cellular biology and neuroscience from Rutgers University. Marissa received her BS in Biochemistry and Molecular Biology from UMass Amherst in 2022. Recent reports revealed that circRNAs are expressed in developmental- and tissue-specific manners and are highly enriched in the nervous system, particularly in synapses. Studying transcription, chromatin and gene expresion in the brain. Anna moved to Boston in 2022 to continue her career as a scientist focused on pediatric neuro-oncology and hopes to make a difference for the young patients affected by this diverse group of devastating diseases. A polar mechanism coordinates different regions of alternative splicing within a single gene. These questions are: Circadian clocks organize cellular, physiological, and behavioral timing in 24-hour cycles. We have also generated stable nuclease-dead Cas9 activator (dCas9a) cell lines that can be used, after transfection with gRNAs, to perform overexpression screens - thus complementing loss of function screens. Derr A, Yang C, Zilionis R, Sergushichev A, Blodgett DM, Redick S, Bortell R, Luban J, Harlan DM, Kadener S, Greiner DL, Klein A, Artyomov MN, Garber M. End Sequence Analysis Toolkit (ESAT) expands the extractable information from single-cell RNA-seq data. Jens joined the Bando Lab in 2023 as a visiting graduate student to understand how distinct histone mutations influence gliomagenesis and also aims to exploit the dependence of pLGGs on the MAPK pathway. High-accuracy determination of internal circadian time from a single blood sample. The current model postulates that circadian oscillators keep time by complex transcriptional and post-transcriptional feedback loops. Sebastian Kadener Prof. Nac. Previously, Kadener was a Professor in the Biological Chemistry department at the Hebrew University of Jerusalem. Our studies on Drosophila gut led us to establish a model of tumor-induced organ wasting that is relevant to cachexia. I am in the Class of 2024 studying neuroscience and biology. In the Bandopadhayay Lab, Michelle is interested in using computational efforts to better characterize pediatric brain tumors. We generated a dual reporter with a destabilized fast folding GFP (dsGFP) protein and a stable RFP reporter. Mezan S, Ashwal-Fluss R, Shenhav R, Garber M, Kadener S ; A new in vivo model of pantothenate kinase-associated neurodegeneration reveals a surprising role for transcriptional regulation in pathogenesis, [Journal Article] Frontiers in cellular neuroscience (IF: 4.300) (2013) Pandey V, Turm H, Bekenstein U, Shifman S, Kadener S High levels of tumor-derived ImpL2 associate with impairment of mitochondrial and muscle function, reduced ATP levels, ovary atrophy, reduction of insulin signaling in peripheral tissues, hyperglycemia, and a decrease of stored triglycerides (TAG) and loss of adipose tissue. * Corresponding authors. We have adapted and optimized the new CRISPR-based technology, prime editing, to generate precise changes into a target genomic location. To overcome the problem associated with the significant reduction of signal strength of dsGFP, we increased post-transcriptional production of the fluorescent protein by including several translational enhancing elements. For loss-of-function, the lines express one or two gRNAs targeting the coding sequence of a gene or genes. snguyen [at]brandeis [dot]edu Michelle Chukwu Research Technician Metazoan MicroRNAs (2018) David P. Bartel CELL Non-canonical functions of microRNA pathway enzymes - Drosha, DGCR8, Dicer and Ago proteins Circadian clocks are remarkably robust: they are able to keep time without timing cues and are resilient to large variations in environmental conditions. Advantages of CRISPRi as compared with RNAi are that the level of knockdown can be more tightly controlled and the system appears to have reduced off-target effects. J. Biol. Avigayel Rabin, Michela Zaffagni, Reut Ashwal-Fluss, Ines Lucia Patop, Aarti Jajoo, Shlomo Shenzis, Liran Carmel and Sebastian Kadener (2021). Fun fact:When not in the lab or the clinic, Jess loves yoga, sour beers, running on the Esplanade, and writing. Towards the end of her time as a PhD candidate, she became exceptionally interested in the intersect between cancer metabolism and oncogenic signaling, particularly the metabolic switches that support and drive adaptive resistance to cancer therapy. S Kadener, P Cramer, G Nogus, D Cazalla, M de la Mata, JP Fededa, . Working under the mentorship of Dr. Beth Lawlor, April focused on understanding how hijacking of normal developmental pathways lead to heterogeneity and cell state plasticity in Ewing sarcoma. Chem. Samar was awarded the Neubauer doctoral fellowship . When she cant search for the perfect tree to plant, she goes on an occasional run (seasonal runner), does some exercise with the Swedish organization Friskis&Svettis, read books (on her own or with her two kids), and decorates her new Boston home. These tools, which are available via. The tagged endogenous proteins can then be isolated for further analysis by MS. To analyze protein-protein interactions or identify components that localize to discrete subcellular compartments, we developed tools based on APEX, an engineered ascorbate peroxidase derived from plants, and BioID, a mutant form of the biotin ligase BirA from E coli and demonstrated their use for in vivo studies. Zaffagni M, Kadener S. NEAT1 is overexpressed in Parkinson's disease substantia nigra and confers drug-inducible neuroprotection from oxidative stress. One of the key reagents to understand protein expression and function are antibodies. Kadener S., Cramer P., Nogues G., Cazalla D., de la Mata M., Fededa J., Werbajh S., Srebrow A. and Kornblihtt A. A Biblioteca Virtual em Sade uma colecao de fontes de informacao cientfica e tcnica em sade organizada e armazenada em formato eletrnico nos pases da Regio Latino-Americana e do Caribe, acessveis de forma universal na Internet de modo compatvel com as bases internacionais. From a number of screens (receptome-wide RNAi screen, miRNA screens, etc. Explore trending content on Musk Viewer . Leslie received her BA in Biological Sciences from Assumption College in 2010, before moving on to complete her PhD in the Program in Experimental and Molecular Medicine at Dartmouth College. More recently in collaboration Andy McMahons lab at USC we have extended this approach to the mouse. UAS-gypsy structure and functional test. Portal D., Lobo G.S., Kadener S., Prasad J., Espinosa J.M., Pereira C.A., Tang Z., Lin R.J., Manley J.L., Kornblihtt A.R., Flawia M.M. Jess hopes to pursue a career as a physician-scientist, taking care of children with brain tumors and other solid tumors and working hard to improve treatments for her patients. To systematically identify secreted proteins involved in interorgan communication and their origins and destinations, we have developed a novel quantitative proximity labeling approach based on TurboID. These studies have allowed us to better characterize what cell types are present in different gut regions and provide us with new powerful data sets with which we can better characterize the transcriptional networks that define lineage relationships among cell types, cell-cell interactions among cell types (i.e. Sebastian Kadener Associate Professor of Biology Research Description Molecular Neurobiology and RNA metabolism Our laboratory is interested for understanding how molecular processes in the brain determine behavior with special emphasis on RNA metabolism. Allada R., Kadener S., Nandakumar N. and Rosbash M. A recessive mutant of, Portal D., Espinosa J.M., Lobo G.S., Kadener S., Pereira C.A., de la Mata M., Tang Z., Lin R.J., Kornblihtt A.R., Baralle F.E., Flawia M.M. He is also contributing to efforts to characterize chromatin complexes as therapeutic targets for pediatric brain tumors. From such genetic screens we have already characterized a number of secreted factors by which organs communicate their physiological state to others: ImpL2/IGFBP, Myostatin/GDF11, Upd2/Leptin, Activin-beta, Upd3/IL6, and Pvf1/PDGF. Sebastian Kadener BIOL 156A 1 Syllabus: Project Laboratory in Biotechnology [ sn . Adam then moved to Boston for his postdoc in the lab of Dr. Yang Shi where he grew interested in identifying epigenetic targets for pediatric brain cancers. S Kadener, P Cramer, G Nogus, D Cazalla, M de la Mata, JP Fededa, . J. Biol. Regulation of alternative splicing by a transcriptional enhancer through RNA pol II elongation. Nucleic Acids Research47(11): e95; 1-16 (2017). One of these translated circRNAs is circMbl. He plans to continue his career as a physician-scientist in pediatric oncology. Statuses. In his spare time, Eric enjoys any combination of the three Bs: Boardgames, Beer and Broncos (Denver). Alternative splicing regulates biogenesis of miRNAs located across exon-intron junctions. Proximity labeling utilizes enzymes that generate reactive radicals to covalently tag neighboring proteins. Articles Cited by Public access Co-authors. His main research interests include the roles of protein homeostasis, RNA metabolism, and intracellular transport in neurodegeneration. An early ancestor in the evolution of splicing: a. Nogues G., Kadener S., Cramer P., de la Mata M., Fededa J.P., Blaustein M., Srebrow A. and Kornblihtt A.R. Belacortu Y., Weiss R., Kadener S. and Paricio N. Transcriptional activity and nuclear localization of Cabut, the, Belancortu Y., Weiss R., Kadener S. and Paricio N. Expression of, Van der Linden A.M., Beverly M., Kadener S., Rodriguez J., Wasserman S., Rosbash M. and Sengupta P. Genome-Wide Analysis of Light and Temperature-Entrained Circadian Transcripts in, Fathallah-Shaykh H.M., Bona J.L. First, we characterized a Drosophila miRNA, miR-263a, that regulates epithelial sodium channel (ENaC) activity to maintain osmotic and ISC homeostasis. Moreover, we also showed that the neural and molecular circadian systems compensate and interact with each other. PANGEA: A New Gene Set Enrichment Tool for Drosophila and Common Research Organisms Contact. Further, we have established new cell lines and methods for screening (primary muscle and neuronal cells, fluorescent protein-tagged cell lines, CRISPR mutant cell lines), and experimental and bioinformatics approaches methods for addressing off-target issues and other sources of false discovery. Fun fact: Dana is a huge Philadelphia sports fan and is mentally preparing to be the only Eagles fan in Boston. We are now using this tool to establish sensors of each signaling pathway involved in gut homeostasis, and will use these reagents to examine pathway crosstalk. In addition to her passion for medicine and science, Jess is also passionate about advocacy for science. and Kadener S. Mathematical Model of the, Kadener S., Menet J., Sugino K., Horwich M.D., Weissbein U., Nawathean P., Vagin V., Zamore P., Nelson S. and Rosbash M. A role for miRNAs in the, Kadener S., Rodriguez J., Abruzzi K. and Rosbash M. Genome-wide identification of targets of the, Kadener S., Schoer R., Menet J. and Rosbash M. Circadian transcription contributes to core period determination in, Kadener S., Stoleru D., McDonald M., Nawathean P. and Rosbash M. , Rosbash M., Bradley S., Kadener S., Li Y., Luo W., Menet J.S., Nagoshi E., Palm K., Schoer R., Shang Y. and Tang C.H. During development, cells have to interact to coordinate proliferation and differentiation to form tissues and organs. Eric joined the Bandopadhayay Lab in 2020 where he is working to characterize driver mutations found in pediatric brain tumors and developing new approaches to treat these children with targeted therapies. Articles Cited by Public access Co-authors. Sebastian Kadener Principal Investigator M.Sc., The University of Buenos Aires, Buenos Aires, Argentina Ph.D., The University of Buenos Aires, Buenos Aires, Argentina skadener [at]brandeis [dot]edu Staff Sinead Nguyen Lab Manager I am interested in studying circular RNA formation. Additional unpublished work from my lab demonstrates that a subset of circRNAs is translated. We showed that miRNAs have a key role in providing robustness to the circadian system both during development and in adults. Uncovering molecular and physiological functions of circRNAs: Circular RNAs (circRNAs) are highly abundant RNAs produced by circularization of specific exons. News. Last but not least, we recently showed that some circRNAs are translated. Prior to coming to Brandeis, Sayan worked in the Bangalore Life Science Cluster, India as a research fellow. In the Bandopadhayay Lab, Maddie is working on efforts to identify resistance mechanisms in pediatric brain tumors. In addition, tumor-secreted Upd3 promotes gut stem cell proliferation locally while activating JAK/STAT signaling in peripheral tissues, leading to wasting, lipid loss, and hyperglycemia. We have several efforts underway to produce new binary expression system reagents by CRISPR-mediated knock-in, including split-GAL4, LexA, and QF. *, Eliaz D.*, Cohen Chalamish S., Tschudi C., Unger R., Hashem Y., Kadener S. and Michaeli S. Pseudouridylation in. Dana received her BA in Biology from the University of Pennsylvania (21). We showed that their translation is mediated by IRES-like sequences, is enhanced in response to starvation and happens in association with membranes and in synapses. We believe that this is reminiscent of changes in ISC proliferation observed in animals that feed infrequently, such as the Burmese python, where ISC proliferation is triggered following a large meal as a result of gut distortion. Fun fact:Riley chose her English name when she moved to the U.S because it means courage. Among the next significant questions to address are how complex phenotypes arise in the context of the whole organism and how the programs regulating these phenotypes are influenced by genetic background and environment. Sher was born and raised in Rawalpindi, Pakistan and moved to the United States for his undergraduate studies. To complement RNAi-based approaches, we are also developing a number of tools based on CRISPR technologies. . During her undergraduate studies, she studied the role of the soluble adenyl cyclase (sAC) pathway in pH homeostasis in Dr. Katie Barotts lab. RNA biology Neurosciences. Harvard Medical SchoolHoward Hughes Medical Institute, Communication between cells, tissues and organs in Drosophila. Furthermore, dehydration of the intraluminal surface in miR-263a mutant guts increases bacterial infection, as evident by the increased expression of antimicrobial peptides. en Rescate y Seguridad, Instructor de Salvamento acutico y RCP, Guardavidas. What are the neural and molecular basis of temperature adaptation and compensation? Contact the author . For doing this, we study the circadian clock from a systemic point of view, including studies at the molecular and neural levels. Bartok O., Teesalu M., Pandey V., Hanan M., Poukkula M., Havula E., Moussaieff A., Vodala S., Nahmias Y., Kadener S.* and Hietakangas V.* Cabut-dependent repressive branch of the sugar sensing transcriptional network regulates glyceroneogenesis. Sebastian Kadener Activities Collapse all Works (11) sort Sort The chromatin factor ROW cooperates with BEAF 32 in regulating longrange inducible genes EMBO reports 2022-12-06 | Journal article DOI: 10.15252/embr.202254720 Contributors : Neta Herman; Sebastian Kadener; Sagiv Shifman Show more detail Source : Crossref Our major contributions to tool development are the Gal4-UAS system, FLP-FRT germline clone technique, in vitro and in vivo genome-wide RNAi and CRISPR screening platforms and resources, and in vivo proximity labeling methods. This work, led by Sebastian Kadener, summarizes years of development of a pipeline that improves upon current tools. Determine the nature of circadian rhythms within organisms. Following our identification of adult Drosophila intestinal stem cells (ISCs), we used this system to characterize genes and pathways important for gut homeostasis and in particular, to analyze crosstalk among signaling pathways. . Are circRNAs involved in neurodevelopmental and neurodegenerative diseases? Lerner I. I am an undergraduate student in the class of 2024 majoring in Neuroscience and HSSP. leptin), neural development, and behavior are conserved, . Selected for Faculty of 1000 (Factor 3 Recommended). We are currently applying pooled screening to identify essential genes, to perform combinatorial synthetic lethal screens, and to screen for resistance or sensitivity to drugs, toxins and pathogens. Biol., The University of Buenos Aires, Buenos Aires, Argentina, Ph.D., The University of Buenos Aires, Buenos Aires, Argentina, Undergraduate Departmental Representatives, Heller School for Social Policy and Management, Rabb School: Graduate Professional Studies, Graduate Professional Studies (Online Programs). Prior to starting graduate school, he spent two years doing research on ALS at Massachusetts General Hospital with Dr. Lagier-Tourenne. In the absence of miR-263a, the intraluminal surface of the intestine dehydrates, ECs swell, and ISCs overproliferate as a result of cytokine production by stressed ECs. Interestingly, circRNAs accumulate in an age-dependent manner suggesting their relevance to age-related homeostasis and/or pathogenesis. Further, our work and that of others has suggested functions of these moleculesin vivo. . Main Research Areas (click to expand headings below for more information). April received her BS in Cellular and Molecular Biology from Beloit College in 2017, where she worked in the lab of Dr. Amy Briggs studying the role offpgsgenes inArabidopsis thaliana. We renamed the center DRSC/TRiP-Functional Genomics Resources to better represent our current capabilities. Simchovitz A, Hanan M, Niederhoffer N, Madrer N, Yayon N, Bennett ER, Greenberg DS, Kadener S, Soreq H. Thermosensitive alternative splicing senses and mediates temperature adaptation in, Reconfiguration of a Multi-oscillator Network by Light in the. Proximity labeling utilizes enzymes that generate reactive radicals to covalently tag neighboring proteins. Sebastian Kadener E-mail (s) skadener@gmail.com Organization name The Hebrew University of Jerusalem Street address SIlberman Institute of Life Sciences, Edmund J. Safra Campus City Jerusalem ZIP/Postal code 91904 Country Israel Platform ID GPL20301 Series (1) GSE154616 Mammalian circRNA Atlas Relations BioSample SAMN15566042 SRA SRX8753063 Not always better more recently in collaboration Andy McMahons lab at USC have... Is a huge Philadelphia sports fan and is mentally preparing to be only! Towards her bachelors degree at Lund University, Sweden to coming to Brandeis, Sayan in. Their linear isoforms, indicating a potentially widespread layer of previously unknown gene.. 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For science from a single blood sample gene expresion in the Class of 2024 studying neuroscience and HSSP of Aires... Age-Related homeostasis and/or pathogenesis we are also developing a number of approaches address... And confers drug-inducible neuroprotection from oxidative stress the mouse 21 ) Rajewsky & # x27 ; s: pol! For loss-of-function, the University of Pennsylvania ( 21 ) transcriptional activators differ in their abilities to control alternative:... Age-Related homeostasis and/or pathogenesis reagents to understand protein expression and function are.... Some circRNAs are expressed in developmental- and tissue-specific manners and are highly abundant RNAs produced by circularization of specific.! By different cell types can regulate blood vessel formation in normal cells and tumors environmental sebastian kadener lab... Of Jerusalem nucleic Acids Research47 ( 11 ): e95 ; 1-16 ( 2017 ) and! 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The Michael Rosbash laboratory developmental- and tissue-specific manners and are highly abundant RNAs produced by circularization of specific.. Reagents by CRISPR-mediated knock-in, including split-GAL4, LexA, and behavioral timing in 24-hour cycles early! Drosophila and Common research Organisms Contact accurate annotation and quantification of circRNAs is translated coordinate proliferation and differentiation: his. His native language of Urdu, Shers full name translates to lion/tiger brave am researching the of. Various cell types can regulate blood vessel formation in normal cells and tumors moved to the U.S because it courage! Covalently tag neighboring proteins molecular and physiological functions of these molecules in gut! Mosquito vectors of infectious diseases Eagles fan in Boston a postdoc working in Bandopadhayay... Cellular biology and neuroscience from Rutgers University in the Bandopadhayay lab, is. 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Recently showed that the neural and molecular basis of temperature adaptation and compensation Mata, JP Fededa.. Mutant guts increases bacterial infection, as evident by the increased expression antimicrobial.